Note: Some of the cell experimental pathways involved in this article are slightly different from the pathway strategies in reality, and the purpose is threefold, one is to avoid leaking the topic of the research group in the file, the second is to avoid professional peers catching insects, and the third is that this novel is more entertainment-oriented, not a paper library. Please be informed.

Basically, the first step in the path of basic medical research is cell resuscitation technology.

Basic medical experiments are experiments that carry out various interventions on tumor cells or other cells from the perspective of cells to observe the characteristics of cell activity, function, reproduction, etc.

The purpose is very simple, that is, I hope that my own treatment can intervene in these cells, which may become a new direction for the treatment of the diseases represented by the cells and drug treatment.

You can't just slap people on them!

"Cells are thawed, washed, cultured. Thaw, wash, and incubate. Fang Ziye first went through the corresponding routine in his mind, and then turned back to Luo Tingzhu and smiled.

But Luo Tingzhu did not laugh at Fang Ziye, but encouraged and raised his fist at Fang Ziye.

To do disease research, you must first have the cells of the corresponding disease. This teacher, Yuan Weihong, has already bought it, and it has been frozen after differentiation.

At the beginning of the cell experiment, a part of the cryopreserved cells is re-recovered, cultured and propagated as the material for subsequent experiments.

Generally, it is cryopreservation with liquid nitrogen.

After removing the cell sample from the frozen state, Fang Ziye immediately took out the cryovial from the liquid nitrogen tank that was bubbling white and like a fairyland, and put it into a water bath preset at 37°C, and gently shook it until the sample completely melted.

The faster this process, the better, because there are some reagents in the cryopreservation solution that can lyse the cells, which are not suitable for cell growth.

If all the cell lines are finished by Fang Ziye, then Yuan Weihong will need to re-purchase them.

It is estimated that criticism will inevitably occur.

Fang Ziye's speed is very fast, after thawing, quickly put the cryopreservation solution and cells into the culture medium, and use the medium to dilute and wash the cryopreservation.

This is like when a person is about to be poisoned by gas, he must go to a place with fresh air to breathe as soon as possible, while detoxifying and avoiding the further invasion of toxins.

Because it is the first operation, Fang Ziye is the best principle, the suspension after diluting the freezing medium is centrifuged and separated once, and then the suspension in the test tube is poured out, and only the adherent cell lines are left, and then it is evenly beaten with the medium again.

Take the suspension and add it to the pre-prepared Petri dish.

"Senior Brother Fang, you are so extravagant! If you were in an internal medicine laboratory, you would be scolded for being so extravagant. Luo Tingzhu smacked his tongue slightly.

Even if the medium is well equipped, because the serum is added to it, it is expensive, and every milliliter can be measured in the yuan of RMB.

Fang Ziye's centrifugal tube may have wasted at least ten yuan.

Because it is more simple and violent, after the cells are removed from the cryopreservation, the cryopreservation solution in the cells is directly diluted with the medium, and some are added directly to the medium.

"The first time, waste a little, so as not to raise the cell line to death."

"After covering the petri dish in this way, shake it for a few days to let it grow naturally for a while, and then the wall is full. You can collect cells, right? Fang Ziye looked back and asked.

The cell lines that Yuan Weihong survived are all treasures, basically the mother plant, and they can be cultured and passed on for several generations before experiments, which is no problem.

The price of this cell line is even more expensive, and Fang Ziye does not dare to waste it.

Raise a bottle first, then cryostore a part, experiment a part, and then follow your own experiments, that is, to deal with the next generations of this mother plant culture.

"Yes, I thought Senior Brother Fang, you just wanted to do this directly? After all, Mr. Yuan's project has a lot of funds and deep pockets. Luo Tingzhu smiled.

Fang Ziye hurriedly shook his head.

"Senior Sister Luo, have I already used almost a hundred yuan today?" Fang Ziye asked again.

Luo Tingzhu examined it: "Petri dishes, as well as Mr. Yuan's high-level pipettes, one or two yuan a piece, plus the culture medium, less than a hundred, but more than seventy." ”

"I can't afford to mess with it!" After Fang Ziye spread the cells evenly, he first took them to the microscope for observation.

After adjusting the focal length, I was relieved to find that the cells in the field of vision were all round and floating in the culture medium, and there were no crooked melons and cracked dates, and there were no swimming bacteria.

It's just that at this time, when Fang Ziye carefully observed the movement of cells for a while, Fang Ziye suddenly found that his knowledge was growing wildly.

“+0.1！”

“+0.3！”

“+0.4！”

Almost half a minute passed, and Fang Ziye's knowledge points suddenly increased by 5 points.

This made Fang Ziye slightly stunned, a little inexplicable.

Luo Tingzhu had already left, and Fang Ziye was also wearing a mask, so his stunned and excited was no one to share.

Put the petri dish into the cell culture incubator, empty the garbage in the cell table, spray it with alcohol again, take out all the experimental equipment, and put it into the refrigerator level and lattice drawer in the laboratory.

Fang Ziye sat on a round bench in the laboratory again, slightly in a daze.

In the distance, Luo Tingzhu was already crawling and didn't know what he was copying, and in the distance, there were some academic doctors and brothers of Professor Deng Yong, tapping on the keyboard, as if they were not very satisfied with the data they were processing.

Farther away, there was an academic master's junior brother, following a senior brother and sister who Fang Ziye didn't know in and out of a certain experimental room, still asking, "Senior brother, what are we going to do next?" ”

At this moment, Fang Ziye can be regarded as finally figuring out some things.

In clinical practice, those diseases and changes that we have seen have long been very mature in the diagnosis, treatment, and surgical plans and processes of diseases.

In the laboratory, so to speak, if there are some directions that others have done, then you can't apply for the project, but everything that you apply for is a new direction and field.

Regardless of whether this direction is used or not, even if it fails to be verified, it is a failure that no one else has verified.

What is a Knowledge Point?

What have you learned? At least Fang Ziye himself thinks so, there may be subtle gains in the treatment and operation of clinical patients, among which the knowledge brought by diagnosis and treatment is expensive, so that you can truly experience this process, there must be immaturity.

And the things in the laboratory, every kind of processing direction, may be completely undone by others.

For example, the cell line that he revived by himself was Yuan Weihong, who had specially treated it with reagents, and it seemed to be a cell line that silenced the HK2 gene, so in such a situation, he even just observed its cell movement.

It is.

New knowledge.

Fang Ziye suddenly seemed to have found the door to a new world, and beyond this gate, there was the world, and it was smoke and rain.

What is Knowledge?

The process of making something new, what you don't know, what no one knows, is considered knowledge......