"Senior Brother Fang, can you borrow your PBS? Mine may be contaminated, my cells were growing fine last time, and after a PBS rinse once, this time they all died. While Fang Ziye was pondering, Luo Tingzhu might go to look at his petri dish again.

PBS buffer is the equivalent of bathing cells, and when cells are growing adherently, they need to be rinsed once after replacing the original medium.

If the PBS buffer is contaminated, that's a bad thing.

Compared to tumor cells and bacteria, that's also a little hellokitty!

And once the cell line is infected, it is more distressing than being injured.

The small cells raised in the laboratory and the corn and sweet potatoes planted in the field by a doctor of agriculture are the same reason. Precious.

Fang Ziye was taken aback when he heard this, and turned his head to look at Luo Tingzhu, who was frowning slightly: "Senior Sister Luo, have you lost the petri dish?" Can you show me? ”

Fang Ziye is like a curious baby.

Fang Ziye has never seen what the polluted field looks like, and Fang Ziye wants to guess and see if the polluted field can increase his knowledge points.

If you could, the lab would be a treasure trove.

Luo Tingzhu nodded solemnly, and he could also understand Fang Ziye's mood: "What about PBS? ”

After the contamination, the possible cause was found and it was necessary to re-order, Luo Tingzhu may have prepared the follow-up experimental process, and now the cells are gone, which is equivalent to the experiment without a subject, and if you want to go out to eat, the restaurant has no reason.

"It's okay with this, Senior Sister Fang, you can use it, and you can seal it when you use it." Fang Ziye hurriedly put on a sterile mask and hat and ran into the cell room.

The reagents used in the laboratory should be sealed and stored with a film after use, otherwise they are easily contaminated by bacteria.

The PBS buffer of Luo Tingzhu may be improperly sealed.

After Fang Ziye came to the cell room, he saw three petri dishes with the label LTZ 10.08 written on them, which means that Luo Tingzhu changed the liquid on October 8, or the cells after re-transfer.

It's the object of cell experiments, don't use it indiscriminately.

Fang Ziye's label is FZY 10.10, which is convenient for everyone to distinguish.

Picking up one and putting it under the microscope, Fang Ziye really saw that the adherent cells had been lysed, and there were various impurities and debris in the suspension.

Because the petri dishes are separated, you must not use an oil mirror, you can't see bacteria, and you can't see other common contamination such as C. cepacia contamination, so you can guess that it is bacterial contamination.

And looking at it this way, Fang Ziye really found that hey, his knowledge points have increased rapidly by as much as 3 points.

It may be that the cell line that Luo Tingzhu is raising at the moment has also been specially treated, and it is a special cell line that is not many in the world, at least there is no mature theoretical system.

Such a cell line, even if it is polluted to death, can also allow Fang Ziye to understand what the state of death and lysis are like, which is also a new kind of knowledge.

It's really full of learning.

Fang Ziye hurriedly changed it again, and then changed it again.

I found that everything I saw was almost the same, and I didn't learn any more.

"Too greedy, too greedy." Fang Ziye could only whisper like this.

When Fang Ziye looked at it like this, he saw Luo Tingzhu, who had also prepared a number of reagents and equipment for cell recovery prepared by Fang Ziye before, and deliberately put PBS and culture medium into a 37°C constant temperature water bath in advance to preheat.

Strive to simulate the normal temperature of the human body to the greatest extent, so that the baby tumor cells can grow strong and happy after recovery, and then become a favorable experimental material for Luo Tingzhu.

"Senior Sister Luo, can I help you lose these things?" Fang Ziye asked.

"Lose it, I've asked my senior brother, it's polluted like this, and it can't be saved. I'm also going to do scratch experiments. ”

"Alas! ~" Luo Tingzhu said a word that was a bit beyond the current experimental progress of Fang Ziye.

Fang Ziye didn't immediately delve into it, but took a curious look at his cell culture dish, and found that the cells were still suspended, and after the bed was not attached to the wall, he also felt that he was too anxious.

After putting it back in the constant temperature cell culture box, Fang Ziye said goodbye to Luo Tingzhu.

Luo Tingzhu didn't need to instruct her to do cell resuscitation, staying here was like a joke, only to increase the pressure......

Fang Ziye only entered the laboratory, not full-time and semi-full-time, therefore, clinical patients, Fang Ziye still has to take care of it, and on Monday's surgery day, Fang Ziye still has to go to see if there is a mobile phone meeting.

On Monday, luck was good.

Although Professor Deng Yong and Professor Xie Jinyuan are still working on the progress of the operation non-stop, Fang Ziye has not deprived himself of the opportunity to take the stage in charge of the patient.

Moreover, in the afternoon operation, one of them belonged to Senior Brother Jiehan, and after the split, Senior Brother Sun Shaoqing had to go to another stage for surgery, so Fang Ziye had two opportunities to be an assistant on stage.

In the first one, Fang Ziye also became an assistant, and the other operations were not touched, all of them were gnawed away by Professor Deng Yong and his own teacher.

Finally, after the split, Fang Ziye was fed by his mentor and first completed an incision to protect his hand.

Yuan Weihong's mood at the moment was still on Fang Ziye's more glorious lintel, and after reading it, he said: "When you enter the laboratory, don't fall behind in clinical operations, and occasionally enter the practice room and operating room." ”

Jie Han said, "Master, I heard from the other senior brothers that Senior Brother Fang went to the practice room for two hours last night, and he didn't go back until after eleven o'clock. ”

"Where did you come from to hear so much?" Yuan Weihong asked sideways.

Jiehan's eyes flashed quickly a few times at this moment, and replied: "Master, I trust you to teach me well, senior brother fed me and posted a few more articles, so among the senior brothers, everyone is more face-saving." ”

"Someone asked me to teach them to write a meta-analysis, but I didn't dare to respond."

Nearly three-quarters of Fang Ziye's scientific research output was done with Jiehan. And Jiehan himself published an article separately, shared an article with Fang Ziye, which has been published, another letter has also been published, and the remaining revised article will be published soon.

This scientific research output can be described as a big deal.

In addition, Jiehan himself has conducted a separate meta-analysis, which is about to be completed, and I also heard that when Jiehan is now in his own practice room, he has collected some past clinical data and done a retrospective clinical research analysis, and he is currently in the process of collecting data.

This scientific research output is simply crazy.

Can that be loved?

"Virtue! ~”

"Is there any progress on the sutures? If so, come and sew it into one? Yuan Weihong looked at the two precious apprentices, and he was also in a comfortable mood and raised his eyebrows.

"Master, there's still a loss left, and I can close the rubber skin with a loss, and the incision is not far away." Jiehan replied like this, as if to reassure Yuan Weihong, his scientific research time has not delayed clinical progress.

Yuan Weihong was instantly in a good mood and shouted: "Itinerant teacher, anesthesia teacher!" ”

The anesthesiologist was playing with his mobile phone, and was yelled at by Yuan Weihong, trembling all over, subconsciously looking at the vital signs, all of them were stable, and he also replied loudly with a big northeast accent, glaring angrily: "What are you doing?" ”

"Let's eat together in the evening!" Yuan Weihong continued to speak loudly.

"Eat, eat, why are you so loud?" The anesthesiologist's voice was quieter.

But immediately, he put his phone away again and whispered: "Viagra, where are we going to eat?" ”

Yuan Weihong was operating on the same side as he discussed a place to eat with itinerant anesthesiologists.

Jie Han carefully pondered the details of Yuan Weihong's operation on the operating table, helping with one hand and changing flexibly with the other, as if pondering.

Fang Ziye felt that the light of the shadowless lamp didn't seem to be so strong, and he felt that it was better than ever.

The main thing is, at the end of the operation.

"Knowledge point +0.4" fluttered, making Fang Ziye feel more and more comfortable.