"Do you prefer clinical?" As the clinic was about to end, Yuan Weihong asked as he watched the smile on Fang Ziye's face bloom happier than ever.

In the past three years, Yuan Weihong has rarely seen his students so happy.

Fang Ziye watched that after he had just improved the basic theory of trauma surgery, the balance of knowledge points climbed to 22.1 again!

He turned his head and said, "Master, I like both clinical and scientific research, and I actually like the process of exploring the unknown. ”

I silently said in my heart that the knowledge points brought by this exploration process are the happiest for me. But over time, I found that this process is actually a kind of enjoyment.

"You're going to the lab at night, aren't you? I won't ask you out to eat. Yuan Weihong looked at the time on his watch, pointing to seventeen o'clock, half an hour before he got off work.

"Well, Master, Senior Sister Luo's transwell chamber experiment, I'm going to take a look at the incubation state today." Fang Ziye nodded, and at the same time looked sideways to look out again, making sure that there were no follow-up patients waiting outside for the time being, so he pushed the door slightly.

It's easier to talk.

The free time of the clinic day before the end of work is actually worth enjoying.

Fang Ziye gave Yuan Weihong a disposable tea cup and another cup of hot water.

"Can you still get that fir fungus last time? I can buy a little. Yuan Weihong suddenly asked.

"Huh? This season is outdated, early October, the last batch, and it's hard to find right now, but I can help you ask, Master. Fang Ziye's eyelids flickered a few times quickly.

It stands to reason that the teacher has something to eat, and such a good teacher has to do a favor by all means.

It's just a seasonal mountain product, which is really not something that can be obtained by trying to find a way, if it is the kind of frozen, it is estimated that the taste is not so good.

"Forget it after that, don't bother, I'm just asking casually. Don't take it to heart. ”

"The thing you have to pay more attention to is to help Tingzhu do a good job in the preliminary research of the application topic, she belongs to the doctoral students who are not within the quota, and Professor Deng Yong did not agree to her to study at the beginning because the project fund is limited."

"The students that Professor Deng Yong originally accepted, plus the new doctoral students, must ensure that they graduate first, and if Luo Tingzhu wants to get more funds to do projects, she has to work a little harder on her own."

"There's no way around it. In fact, if she stays in internal medicine, it's fine. Yuan Weihong changed the subject.

Fang Ziye said with a smile: "Senior Sister Luo may have taken a fancy to the strong muscles of our orthopedic teachers, so she came to our trauma surgery. ”

Yuan Weihong was stunned when he heard this.

Then he touched his watch in a trance and sighed: "If you are an ordinary child from your original family, sometimes beauty is not lucky, but also an original sin." ”

"This is life, chicken feathers."

This topic is too melancholy, it is difficult for Fang Ziye to empathize, his own family is relatively happy. Now my parents are willing to support about 2,000 yuan a month for living expenses.

"Oh, by the way, Ziye, if you have good cell experiment technology, all the experimental processes can be solved once or twice, and we can save money on the funds, and send you and Jiehan some labor fees." Yuan Weihong winked at Fang Ziye.

Fang Ziye was thoughtful, and his eyes rolled around.

No matter what kind of project funds, it is a normal, legal, reasonable and compliant expenditure to pay a master's student, such as three to five thousand labor fees.

It's just a general budget, and it won't have more spare money. After all, not every experimental process for everyone can be done in one step.

It takes several times to succeed, unless the proficiency is very high.

However, Fang Ziye felt that he might save a little more money on experimental consumables, so it was really possible to get two or three thousand labor fees a year.

It's just that such a horizontal comparison is really a drought that is drought, and a waterlogging is dying.

"Thank you, Master." Fang Ziye is not rich, so he doesn't dare to have extra ideas.

Yuan Weihong shook his head: "Hmm! ”

"Funds should be used well, careful budgeting, the place that should be saved can be saved, but should not be saved, must not be saved, strictly ensure the principle of sterility, although the pipette is expensive, but it should be used to ensure the rigor of the experimental process."

"Saving money is not so saving, the most important thing is to make the results of this subject, and slightly push the door of science and lose it, this is what we should do with the money given by the state."

"What's more, Jiehan's scientific research background is very deep now, and when he applies for municipal and school-level projects, his background is similar to yours, so he may get a little more money for our team in the future."

"One is the project funding, the other is the number of projects, which are all good things."

"Money is the only way to do things." Yuan Weihong said to Fang Ziye, and he couldn't say anything to the outside world.

……

At the end of the outpatient clinic, Fang Ziye did not delay much, and after helping Yuan Weihong put the white coat, he went directly to the laboratory.

Although Professor Deng Yong's team is large, Luo Tingzhu is not special, so she has no funds to do experiments, and she needs to work her own.

So Fang Ziye, as a member of Yuan Weihong's team, naturally try to help if he can, otherwise, Yuan Weihong will also come to the laboratory in person.

And, when Fang Ziye came to the laboratory, he found that Luo Tingzhu had been staying in the laboratory from two o'clock in the afternoon to six o'clock in the afternoon.

More worried than anyone else about the progress of the experiment.

She didn't have much money to do the preliminary experiments for the application project, so she couldn't afford the slightest waste and carelessness.

"Senior Brother Fang, I think this petri dish is almost countable, can you take a look?" After Luo Tingzhu saw Fang Ziye arrive, he quickly stood up, and at the same time prepared the sterility examination gloves and disposable suit masks for Fang Ziye.

"Well, okay, have you eaten?"

"Shall we order a takeout first?" After Fang Ziye came in, he asked.

"It's already ordered, senior brother." Luo Tingzhu nodded, indicating that his logistics work was very well done. I only think about cell babies and experiments.

After the Transwell experiment, a cell count is performed to represent the aggressive ability of tumor cells with objective data.

Since this is a preliminary experiment, only direct counting is required.

Then, even if it is a direct counting method, it still requires certain experimental steps to react relatively objectively -

Fix the cells first and don't let them move again.

Carefully remove the chamber with forceps, blot the upper chamber medium, and gently wipe the cells in the Matrigel and chamber with a cotton swab. A new 24-well plate was added with 600 μL of 4% paraformaldehyde, and the chamber was placed in place and fixed for 20-30 min.

Then do the cell staining!

Remove the chamber, blot the upper chamber fixative solution, and transfer to the well with about 800 μl of 0.1%-0.2% crystal violet staining solution pre-added and stain for 15-30 min. Remove the crystal violet that does not bind to the cells, gently wipe the upper side of the chamber with a cotton swab and wipe off the dye that binds non-specifically to the upper surface of the chamber for subsequent microscopic counting......

Fang Ziye is very much looking forward to this step, because whether it is someone else's cell experiment or his own, as long as he discovers it and participates in the experiment, he will definitely have the benefit of knowledge points!

The two of them moved to the microscope...

*Note: Some of the experimental steps involved in this article, some of them are simplified versions, mainly put them up completely, those who understand understand it, most people don't understand it, considering the feelings of most book friends, you can read it roughly.

Summary: The transwell experiment is to detect the invasion ability of tumor cells, and the ability to invade is indicated through technology, which is not simple, but it is a tool to prove the ability.