Microscopically, the number of cells that have penetrated through the membrane is quite impressive.

If we compare the normal group, the number occupies a certain advantage, and it has been stained, and it can be seen that the cells treated by Luo Tingzhu are more aggressive than the normal group.

This phenomenon means that the cell babies treated by Luo Tingzhu are more aggressive than normal cell babies.

"Knowledge +28.6!"

When the huge amount of knowledge points increased, Fang Ziye was quite shocked and at the same time, he felt that it was reasonable.

Although it is only a preliminary experiment and has not become a systematic topic, it is also a new direction and represents a breakthrough certification knowledge.

"Senior Sister Luo, your early experiments will only do this step of transwell, right?"

Luo Tingzhu's attention at this time was completely in the mirror, and the tone of his reply was slightly emotional: "There is no money to continue to do it?" ”

But after speaking, she realized that this tone was not right with Fang Ziye, after all, she had no money to do experiments, and she had nothing to do with Fang Ziye.

"Senior Brother Fang, thank you~" Luo Tingzhu turned his head and smiled, most of his smiles were blocked, but when his big eyes and eyelashes moved, he was full of kindness, which meant that their owners were very happy at the moment.

"It's okay, I have to thank you for giving me the opportunity to practice, I didn't do anything bad after all."

"Then junior sister, you can count the statistics first, and I'll go and see my resuscitated cells first." Fang Ziye didn't continue to put pressure on Luo Tingzhu, glanced at it twice, and didn't see any more increase in knowledge, so he decisively switched to the cell culture incubator.

Each step of the experimental process, the cultured cell line, is the original material of the experiment, once entered the experimental group, the previous cell line becomes the experimental material, can no longer be used, needs to be disposed of, resuscitation or culture again.

After Fang Ziye did the scratch test and proved that HK2 does play a certain role in the metastasis of giant cell tumor of bone, the next thing to confirm is the promotion of the aggressiveness of HK2 on giant cell tumor of bone.

Luo Tingzhu continued to observe and count the cells, preparing for the application project.

The preliminary experiment is only a part of the experiment, mainly to express that I have done a part of the experiment, which confirms the feasibility of my application for the topic, and now I can get the result with only money.

However, due to the limited financial ability of individuals, they cannot support the further progress of their own projects, and they need to be supported by the project fund team at the university, municipal, provincial or even national level.

If you don't have money, you can do the project on your own, which is tantamount to a fool's dream.

No matter what kind of experiment, the materials for the experiment, such as cells and rats and rabbits to raise, are more than the monthly cost of the graduate student himself, so how can he afford it unless he has a lot of money?

Generally speaking, many colleges and universities, when introducing talents, will mention a large amount of scientific research start-up funds, which is so that you can have money to prepare for the preliminary research of applying for large-scale projects.

It's very necessary to study the start-up capital, otherwise you won't be able to enter this temple.

It is difficult for a good woman to cook without rice.

Luo Tingzhu, in fact, was also transferred by Professor Deng Yong to him a lost research start-up fund, so that she was able to make the current preliminary research, and then in the name of Professor Deng Yong or whom, apply for a small project, get money, do some basic experiments, and accumulate her resume, in order to prove her scientific research ability and prepare for staying in the hospital.

Compared with Luo Tingzhu, Fang Ziye is like this, looking for a young tutor, a master's student who does not occupy any advantage in finding a job, once he can stay in the college for a doctorate, then the young tutor has applied for the general project fund, or the youth fund, which is the economic pillar that Fang Ziye can make a big difference.

As long as Fang Ziye can produce output, the funds in the subject can be arbitrarily disposed of.

Fang Ziye watched the cell baby group that he cultivated again, after knocking out the HK2 gene, the small reptiles with adherent cells growing only on the wall grew extremely slowly.

Originally, the growth rate of giant cell tumor of bone was not fast, and it is estimated that this batch will have to wait about ten days for it to grow full, and then the next round of experiments can be carried out.

If the experiment fails, then another batch is cultivated, and it is another ten-day cycle.

Many academic graduate students, whether they are master's or doctorate, are hoarded in this way most of the time.

After Fang Ziye looked at the cells he had cultivated again, he hesitated a little for the sake of insurance, and decided to change his skills related to cell basic experiments from level 2 to level 3.

Whether it is a scratch test or a Transwell invasion test, it is the simplest component of the cell experiment group, and then the subsequent flow cytometry and other experiments are complicated and need to deal with a lot of processes.

Moreover, Fang Ziye's own experimental time is not completely abundant, so it is natural to be safe.

After the addition is completed, the large amount of knowledge points obtained today has been almost emptied again, and the balance of knowledge points is only 0.8.

When I came back from the outpatient clinic, it was 22.1, the results of looking at the cells increased by 28.6, and the experimental operation increased by 0.1 knowledge points, so that I could have made up 0.8, otherwise it would have been only 0.7.

The knowledge points are not enough, not enough at all, and the income of 40 to 50 points a day is still not enough.

Fang Ziye saw Luo Tingzhu "click-click" and took pictures many times, recording various data from the previous experiments, and estimated that the field of vision recorded was definitely more than three or five.

"Senior Sister Luo, in fact, there is no hurry, the application time for school-level projects is generally December and June, and the municipal and provincial projects are behind the general project application, which is April and May." Fang Ziye was relieved.

"Well, you have to try it, if you can only win the school-level project, it will only be 20,000 yuan at most, and it is very reluctant to complete this small path, if you can get the city-level project, there may be about 50,000 yuan, then it would be good." Luo Tingzhu looked forward to it.

At the same time, he carefully put away his petri dish again, as if to avoid his data record being imperfect, and when the time comes, there will be a shortage, and after eating, he will have to continue to take a few more photos for the bottom line.

Next, she had to statistically process the data from the invasion experiment.

It's hard for everyone.

If Fang Ziye's mentor was not Yuan Weihong, but Deng Yong, it might not be as difficult as Luo Tingzhu, but it was not easy.

After all, the choice is a double edge!

The two of them left the cell room before and after, and after the disinfection and sanitation were done, they also returned their shoes to the right, and then changed their shoes, washed their hands and disinfected, and waited for the arrival of the takeaway.

After dinner, Fang Ziye didn't have much to do, and went to the practice room first, while Luo Tingzhu continued to stay in the laboratory, as a little reptile who was working hard to process data and apply for the subject.

Fang Ziye was in the elevator room, looked at Luo Tingzhu sideways, and could only pray silently for her in his heart.

The project fund applied for by the teacher is the teacher's public property within the division, and it is almost impossible for people who have no student relationship with the teacher to get a dime, which is the difference between the scientific research group and the large group.