Hard

Almost unlikely.

After seeing this information, the three young postdoctoral fellows instantly retreated, after all, Xu Mang's proposal to solve the problem of replacing a certain DNA fragment with a target sequence is a false proposition in the world, it does exist, but it cannot be realized.

"Don't you think it's difficult?" Xu Mang saw the expressions of the three of them, and knew that they might feel that they were crazy and made such a request, but these requests were made by others, and then said helplessly: "This is a request issued by the Academy of Sciences, so... Let's do our best. ”

Suddenly

The three of them didn't have any words, and silently accepted the difficulty in front of them that could not be completed, if it wasn't Xu Mang standing in front of them, they might have left, but it was Xu Mang standing in front of them, and they felt that there might be a chance to do so.

"Professor Xu?"

"What kind of approach do we take?" asked one postdoc, "is it the same AI model as before, using artificial intelligence to give new life to gene editing research?"

Xu Mang shook his head and said indifferently: "I don't know... Perhaps, but we need to first complete the theoretical framework for the problem of replacing a DNA fragment with a target sequence, and this problem is not the only problem we need to solve, and new problems will be encountered at any time. ”

And the new ones?

This...

The problem of replacing segments with target sequences was already outrageously difficult, and it turned out that it was just one of many.

Finally got to work,

However, it never occurred that the so-called starting work was to repeat the experiments, from the CRISPR–Cas9 system, and only record the process of each failure, and Xu Mang was responsible for checking for these errors.

No way

This is the best way Xu Mang can think of, through the comparison of the wrong process, from which to find out the starting point, a day or two is quite stable, but as time slowly fades, everyone's enthusiasm is gradually exhausted, like a picture of the walking dead.

On this day,

It's still less than five days before the school holidays,

Postdoc Xiao Chen came to the laboratory, he was going to ask Xu Mang for leave in advance today, the Spring Festival was about to begin, and he had to go home early to avoid the peak of the Spring Festival, but when he told his thoughts to the other two colleagues, he found out... The three of them had the same idea.

“？？？”

“？？？”

“？？？”

Xiao Chen said with a confused expression: "The three of us... Do you want to go back? What should I do with this project? Let Professor Xu Mang stay alone in the laboratory? Although he is half from the Demon Capital, is it too cruel to him?"

"What should I do then?" Xiao Shen smiled bitterly: "You can't help but go home, right?"

"yes. ”

"After all, it's the Spring Festival, I should go back, I haven't been back for a year. Postdoc Xiao Qian said: "Actually, this problem... It won't be solved for a while, we've been doing it for a week, and we're still standing still, so it's better to relax and change our mood. ”

Then

Xu Mang carried a bag of fried buns and two bags of soy milk, slowly came to the laboratory, saw the three of them sitting together chatting, and asked with a smile: "Have you had breakfast? I bought a lot of fried buns, why don't you eat a little together?"

Seeing Xu Mang's arrival,

The three of them didn't know how to speak for a while, and finally Xiao Chen said, "Professor Xu... I'm going to ask you for leave today, I'm going home early to avoid the army of the Spring Festival, I haven't been back for a year..."

"Professor Xu, I also want to take a leave of absence. ”

"Me too. ”

In the face of the three people's leave, Xu Mang was not surprised, and nodded with a smile: "All approved, of course I have to go back for the New Year, right..."

Words fall,

Xu Mang took out three red envelopes from his shoulder bag, handed them to the three of them, and said seriously: "I was going to give it to you later, but since you are going home today, then I will give you the red envelopes now." ”

Suddenly got Xu Mang's red envelope, and the three of them were surprised for a while, but they were even more surprised that later, this red envelope was a little exaggerated, and it turned out to be a full 10,000 yuan.

"This..."

"Professor Xu?"

"This red envelope is a bit exaggerated!" Xiao Chen said with a serious face: "We can't accept it." ”

"Take it. ”

"I don't know how much money is in it, it's all prepared for me by my wife. Xu Mang said indifferently: "Take it, take it, it's okay... Whoever dares to talk nonsense, let him come to me directly and confront me face to face. ”

Looking at the three parting people,

Xu Mang returned to the empty laboratory again, maybe this is his fate, the fate of fighting alone, sitting in the office, silently smoking a cigarette, thinking about the future, thinking about how to face failure or success.

I have been studying the problem of replacing a DNA fragment with a target sequence for more than a week, and I have been wandering in a maze without finding any way out, and the process of seeking the truth has been so bumpy, and the reality has become even more cruel.

After thinking about it for a while,

Xu Mang decided to go outside for a walk, and staying in the office for a long time would break his nerves.

On campus,

Xu Mang walked all the way to the school gate and saw that many students were ready to go home, but the scene in front of him made Xu Mang a little melancholy, came to a café that had not yet closed, ordered himself a cup of coffee, sat in a window position, and looked at all kinds of people on the side of the road.

maybe

On the day of birth, everyone didn't know that it was actually an unreasonable world.

Some people's dreams are the stars and the sea, some people's dreams are a house, some people are born with talents, and some people are dying by sweat... You can't lose at the starting line, but some people have been standing at the finish line.

This is the reality, this is the reality that there is a huge gap, and finally accept that you are just an ordinary person.

Resigned to fate?

However...

What Xu Mang sees is hard work, and living in this unreasonable world itself requires a lot of courage.

"Hello?"

"Wife?"

"I'm in... Cafe. Xu Mang received a call from Xiao Man and said with a smile: "It's not... Everyone has gone home for the Chinese New Year, and I am left alone in the laboratory, so what can I do? I can only sit outside and drink coffee. ”

“...”

"Let's go home. Yang Xiaoman suddenly fell into deep thought, and said seriously: "Husband... Will you go back to your house for the New Year this year or Chinese New Year's Eve, and then return to the magic capital on the third day of the new year?"

"Hmm!"

Then

Yang Xiaoman squeaked and asked, "What kind of gift to buy?"

"No, you don't. ”

"My parents just need to see you coming, you don't need any gifts. Xu Mang said indifferently: "Or... Buy some jewelry and get some tea for my dad. ”

"Hmm!"

"I'll do it right away. ”

syllable

Hang up directly,

Xu Mang just finished drinking a cup of coffee, silently returned to the laboratory, and began his clueless day, until noon, Xu Mang woke up from this muddy state, ate a not so delicious lunch, and continued to start the afternoon of the soul.

Boil, boil, boil,

This day was mixed up by Xu Mang, he really couldn't do anything, he originally wanted to rely on the system to solve it, but found that this time the system gave restrictions, and he could only rely on himself to solve it, and what was even more excessive was that he accidentally triggered the task and asked to solve it before the New Year.

...

"?"

"How... Why is there no one?"

Early in the morning,

Xu Mang walked on the way to the laboratory and found that the school was empty.

Sitting on the chair in the office, I took out the experimental data of the previous three postdoctoral fellows, this is the N+1 time to repeat the comparison, Xu Mang has not made any progress before, and this time it is about the same, but it has become a habit.

"Perhaps..."

"I'm thinking wrong. Xu Mang frowned, took out his notebook from his satchel, connected to the Internet, and opened the database of the Institute of Biochemistry and Cell Biology of the Academy of Sciences.

"Huh?"

"Wait a minute!"

"Why do I keep staring at the problem of replacing DNA fragments with target sequences?" Xu Mang suddenly came to his senses, and he found that he was a little too iron on the issue of replacing DNA fragments with target sequences, and this problem could be completely bypassed.

An immature idea came to me, first cutting off one strand of DNA and inserting the target sequence Y, then the original sequence was cut off, after cutting off another strand of DNA that had not been edited, and finally the CRISPR system repaired the severed strand of DNA.

This was Xu's initial idea, and like the CRISPR–Cas9 system, Cas9 was used to identify specific DNA sequences, but Cas9 was modified to cut off only one strand of DNA, and then reverse transcriptase was guided by the RNA strand to perform gene editing at the cut.

"There it is!"

"I gradually have a feeling!" Xu suddenly gained a new life, but this feeling of rebirth only lasted for about ten seconds, and it was dumb in an instant, if you follow this line of thought, it seems that you need a lot of research experiments, but you don't know how to study biology.

Looking for Xiaoman?

Xiao Man studied economics and knew how to fart biological experiments.

"What should I do?"

"Or... Looking for Academician Zhong?" Xu Mang took out his mobile phone and dialed the number.

honk

called out,

Xu Mang hung up directly.

Shouldn't this be the time for 'Xu Man System No. 2' to appear?

...

night

Always come quietly,

Yang Xiaoman had just thrown all his clothes into the washing machine and was about to go back to the living room to watch TV, but found that Xu Mang, who should have been paralyzed on the sofa, was gone.

"What's the situation?"

"What about this guy?" Yang Xiaoman frowned, thinking that he seemed to be struggling with the progress of scientific research recently, could it be... Have you found a solution to your problem?

tiptoed to the door of Xu Mang's study, and gently opened a crack in the door, through this slight gap, Yang Xiaoman found that Xu Mang was sitting in front of the computer, typing on the keyboard seriously, and after a while, he lowered his head and picked up the pen, not knowing what he was calculating on the paper.

Xiao Man silently closed the door, helplessly with a hint of love.

The big bad guy who grinds people!

......